FIELD: medicine.
SUBSTANCE: claimed invention can be used in microbiligical industry for obtaining recombinant strains of fungi Acremonium chrysogenum - superproducents of cephalosporin C. Plasmid contains expression cassette, which consists of promoter of Aspergillus nidulans glyceraldehide phosphate dehydrogenase gene, terminator of Saccharomyces cerevisiae phosphoglycerate kinase gene, separated by polylinker site. As dominant selective marker it contains gene of Escherichia coli hygromycin-B phosphotransferase under control of promoter trpC Aspergillus nidulans, which determines stability to hygromycin of recombinant strains of mycelial fungi, transformed by plasmid pZEN16 and its derivatives.
EFFECT: claimed invention makes it possible to receive unique vector for agrobacterial transformation of Acremonium chrysogenum, which makes it possible to realise constitutive expression of target genes, responsible for biosynthesis and transport of cephalosporin C.
1 dwg, 8 ex
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