FIELD: chemistry; biotechnology.
SUBSTANCE: transformation is carried out with the use of auxotrophic strains (for example, auxotrophic on uracil), which belongs to the genus Mortierella as the host strain. In particular as a marker is used transmissive to the host-strain a gene, that restores proto-trophicity (stage of the transfer of the gene). Further the transformant is taken away using the restoration of proto-trophicity as a marker (selection stage). As the lipid-producing mushrooms, for example, it is possible to use mushrooms of the species M. Aplina.
EFFECT: effective and rational conducting of the selection of lipid-producing mushrooms without using antibiotics.
6 cl, 3 ex, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| PROMOTER EXHIBITING HIGH EXPRESSION ACTIVITY IN MORTIERELLA MICROORGANISMS | 2014 |
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| HOMOLOGUES OF GLYCEROL-3- PHOSPHATE ACYLTRANSFERASE (GPAT) AND THEIR USE | 2008 |
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| NEW GENES OF LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE | 2008 |
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| NOVEL LYSOPHOSPHOLIPID-ACYLTRANSFERASE | 2010 |
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