FELAMENTOUS FUNGI CELL WITH PROTEASE DEFICIT AND METHODS OF ITS APPLICATION Russian patent published in 2018 - IPC C12N1/15 C12N15/80 C12N9/58 C12P21/02 

FIELD: biotechnology.

SUBSTANCE: cell of Trichoderma for the preparation of heterologous polypeptide includes at least three endogenous proteases with eliminated activity and recombinant polynucleotide encoding the heterologous polypeptide. The cell has an eliminated activity of at least protease gap1 SEQ ID NO: 118 and at least two additional proteases, isolated from the pep2 SEQ ID NO: 182, pep3 SEQ ID NO: 17, pep4 SEQ ID NO: 37, pep5 SEQ ID NO: 58, pep8 SEQ ID NO: 507, pep11 SEQ ID NO: 522, pep12 SEQ ID NO: 530, tsp1 SEQ ID NO: 66, slp1 SEQ ID NO: 82, slp2 SEQ ID NO: 98, slp3 SEQ ID NO: 166, slp7 SEQ ID NO: 231, pep1 SEQ ID NO: 1 and gap2 SEQ ID NO: 129. The polypeptide is produced at a level that is at least twice the level of production of the polypeptide in the corresponding parent Trichoderma cell, in which the proteases do not have the eliminated activity. A method for enhancing the stability of a polypeptide comprises of cultivation of the above cell so that the heterologous polypeptide is expressed. The method for producing a heterologous polypeptide comprises of cultivation of the above cell so that the heterologous polypeptide is expressed, of purification of the heterologous polypeptide.

EFFECT: heterologous polypeptide shows increased stability as compared to the heterologous polypeptide obtained in the corresponding parent Trichoderma cell, in which the proteases do not have the eliminated activity.

23 cl, 55 dwg, 81 tbl, 23 ex