ACYLAMIDASE ENZYME, DNA FRAGMENT CODING SAID ENZYME, AND BIOCATALYTIC METHOD FOR SYNTHESIS OF N-SUBSTITUTED ACRYLAMIDES USING ACYLAMIDASE Russian patent published in 2012 - IPC C12N9/80 C12P1/04 

Abstract RU 2439154 C1

FIELD: chemistry.

SUBSTANCE: invention discloses acylamidase enzyme AA37 from Rhodococcus erythropolis 37 All-Russian collection of industrial microorganisms Ac-1793, with a sequence given in the description. A nucleotide sequence which codes this enzyme is defined. The invention describes a method for synthesis of N-substituted acrylamides in aqueous medium from acrylamide and amines in the presence of an acylamidase biocatalyst in isolated state or in a composition with E.coli cells.

EFFECT: invention enables to obtain N-substituted aliphatic acrylamides from acrylamide and primary aliphatic amines in an aqueous medium.

3 cl, 14 ex

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RU 2 439 154 C1

Authors

Lavrov Konstantin Valer'Evich

Larikova Galina Andreevna

Janenko Aleksandr Stepanovich

Dates

2012-01-10Published

2010-12-03Filed