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2 441 019

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IPC

C07K1/36(2006-01-01)

C07K14/635(2006-01-01)

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Application

2010108327/10, 2007-12-06

(24)

Start date

2007-12-06

(22)

Actual filing date

2007-12-06

(45)

Published

2012-01-27

(72)

Inventor

Sankararaman UmaMaiti DipanvitaSankarankutti MeeraShekkhavat RakeshKopparapu Narishima KumarZaidi GulnazRatod BipinchandraTkhakar Niren PrafulTkhakur PritiChutke AndzhaliMishra Shrikant

(73)

Holder

Jusv Limited

METHOD FOR SYNTHESIS OF RECOMBINANT HUMAN PARATHYROID HORMONE Russian patent published in 2012 - IPC C07K1/36 C07K14/635

Abstract RU 2441019 C2

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology, particularly production of recombinant proteins in Escherichia coli, and can be used for synthesis of human parathyroid hormone (rhPTH (1-34)). It involves constructing cDNA coding a fusion nucleotide ORF under control of an inducible promoter selected from a group consisting of ara_BAD, trp, T7, lac, Pho and trc, by cDNA amplification be means of RT-PCR with using gene-specific primers coding the rhPTH (1-34) fusion protein in which a fusion partner essentially consists of 41 amino acids produced of 124-164 amino acid of beta-galactosidase peptide (LacZ) Escherichia coli. Then the fusion protein is expressed in the presence of lactose as an inducer within 10-30% of total weight-based protein.

EFFECT: invention provides simplifying and reducing the price of rhPTH (1-34) synthesis ensured by optimising its expression in Escherichia coli.

12 cl, 15 dwg, 2 tbl, 16 ex

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RU 2 441 019 C2

Authors

Sankararaman Uma

Maiti Dipanvita

Sankarankutti Meera

Shekkhavat Rakesh

Kopparapu Narishima Kumar

Zaidi Gulnaz

Ratod Bipinchandra

Tkhakar Niren Praful

Tkhakur Priti

Chutke Andzhali

Mishra Shrikant

Dates

2012-01-27—Published

2007-12-06—Filed