NUTRIENT MEDIUM BASE FOR LACTOBACILLI STRAIN SELECTION BY HISTAMINE REDUCTION IN CULTURE MEDIUM Russian patent published in 2012 - IPC C12N1/20 C12Q1/04 

FIELD: medicine.

SUBSTANCE: nutrient medium base for lactobacilli strain selection either by histamine reduction in a culture medium, or by histamine gain reduction in the culture medium represents a sterile deoxidated casein lactoserum containing copper (II) cations in amount 30-1000 mcmol/l, histamine in amount 200-800 mcmol/l. Said nutrient medium base has pH 6.5-7.4. Serum is deoxidated chloride casein lactoserum, deoxidated lactic casein lactoserum or their mixture. In addition, the base can contain target additives for preparing liquid, or semi-liquid, or solid nutrient medium in amount 0.2-300 g/l. When culturing a passed Lactobacillus acidophilus KAA strain version, the histamine level in the nutrient medium makes 485.9±195.1 mcmol/l, Lactobacillus acidophilus NKJC strain - 69.2±28.5 mcmol/l, Lactobacillus acidophilus JCH strain - 129.6±48.4 mcmol/l.

EFFECT: invention enables lactobacilli strain selection by histamine reduction or by histamine gain reduction in the differential diagnostic high-histamine nutrient medium during passing these strains.

2 cl