METHOD OF EVALUATING THE EFFECTIVENESS OF NON-AGGREGATE PREPARATIONS AFFECTING THE METABOLISM OF ARACHIDONIC ACID BY INHIBITION OF CYCLOOXYGENASE-1 BOTH INDIVIDUALLY AND JOINTLY WITH ADP P2Y RECEPTOR ANTAGONIST ON PLATELET MEMBRANES OF THE BLOOD TO THE PATIENT RECEIVING NON-AGGREGATE PREPARATIONS Russian patent published in 2012 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely, the clinical laboratory. The essence of the method for evaluating the effectiveness of non-aggregate preparations on the human body is that the platelets in the blood examined by flow cytometry, carried out not earlier than 5 days after starting taking non-aggregate preparations. Determine the quantitative values of platelet receptors GP IIb-IIIa and P-selectin in whole blood before and after addition of inducer of platelet activation 10 mkM ADP. A changing in the number of platelet receptors for fibrinogen glycoprotein GP IIb-IIIa, K1, as well as changing in the expression of P-selectin on the surface of platelets in whole blood, K2, determined by the developed formulas. If the value of K1 ≤ 10% and K2 ≤ 50%, estimate the effective action of the non-aggregate preparations on the state of the platelets in patient's blood.

EFFECT: using the inventive method allows to increase sensitivity and accuracy of evaluating the effectiveness of of non-aggregate preparations affecting the metabolism of arachidonic acid by inhibition of cyclooxygenase-1 both individually and jointly with ADP P2Y₁₂ receptor antagonist on platelet membranes of the blood to the patient receiving non-aggregate preparations, as well as simplifying the method and shortening the time on it.

6 ex, 1 dwg