FIELD: medicine.
SUBSTANCE: invention represents method of obtaining 4-hydroxyisoleucine or its salt, including stage of obtaining 4-hydroxyisoleucine by hydroxylation of isoleucine or its salt in presence of L-isoleucinedioxygenase, obtained from bacterium belonging to genus Bacillus, and producing 4-hydroxyisoleucine from isoleucine. Invention also relates to L-isoleicindioxygenese, DNA fragment, which codes it, method of its obtaining and application in method of 4-hydroxyisoleucine obtaining.
EFFECT: invention allows to obtain 4-hydroxyisoleucine with high degree of efficiency.
19 cl, 20 dwg, 14 tbl, 9 ex
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