FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and is a method of producing (2S,3R,4S)-4-hydroxy-L-isoleucine or its salt, involving a step for reacting isoleucine in aqueous solution in the presence of bacteria which initially has L-isoleucine dioxygenase activity and 4-hydroxy-L-isoleucine dehydrogenase, and extraction of the synthesised (2S,3R,4S)-4-hydroxy-L-isoleucine. The said bacteria is modified such that 4-hydroxy-L-isoleucine dehydrogenase activity in the said bacteria is disrupted.
EFFECT: invention simplifies the method of producing (2S,3R,4S)-4-hydroxy-L-isoleucine.
6 cl, 4 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF 2-AMINO-3-METHYL-4-KETOPENTANOATE MANUFACTURE | 2007 |
|
RU2392323C2 |
| METHOD OF BUILDING (2S,3R,4S)-4-HYDROXY-L-ISOLEUCINE PRODUCING BACTERIA, (2S, 3R, 4S)-4-HYDROXY-L-ISOLEUCINE PRODUCING BACTERIA AND METHOD OF PRODUCING (2S, 3R, 4S)-HYDROXY-L-ISOLEUCINE OR SALT THEREOF | 2007 |
|
RU2395580C2 |
| BACTERIUM PRODUCER OF PRODUCT OF REACTION CATALISED BY PROTEIN SHOWING 2-OXOGLUTARATE-DEPENDENT ENZYME ACTIVITY, AND METHOD FOR PRODUCING SUCH PRODUCT | 2008 |
|
RU2444568C2 |
| METHOD OF PRODUCING 4-HYDROXY-L-ISOLEUCINE | 2007 |
|
RU2402608C2 |
| METHOD OF OBTAINING HYDROXYLATED L-LEUCINE AND BACTERIUM, TRANSFORMED WITH DIOXYGENASE-ENCODING DNA | 2011 |
|
RU2468085C1 |
| NEW ALDOLASE, DNA CODING ALDLASE, CELLS TRANSFORMED BY DNA, METHOD OF ALDOLASE OBTAINING AND METHOD OF OBTAINING OF 4-HYDROXY-L-ISOLEUCINE (VERSIONS) | 2006 |
|
RU2338784C2 |
| METHOD FOR PREPARING L-AMINO ACID BY FERMENTATION METHOD, STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-AMINO ACID (VARIANTS) | 1999 |
|
RU2207376C2 |
| METHOD OF PRODUCING 4-HYDROXY-L-ISOLEUCINE OR ITS SALT | 2006 |
|
RU2355767C2 |
| SMALL SUBUNIT OF ISOZYME III AND ISOZYME III OF ACETOHYDROXYACID SYNTHETASE FROM ESCHERICHIA COLI, DNA FRAGMENT (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-VALINE (VARIANTS) AND METHOD FOR PREPARING L-VALINE | 2000 |
|
RU2209246C2 |
| METHOD OF OBTAINING L-AMINO ACIDS USING BACTERIA OF FAMILY ENTEROBACTERIACEAE | 2007 |
|
RU2364628C2 |
