FIELD: chemistry.
SUBSTANCE: disclosed is a system of modulating expression of a nuclear receptor-based inducible gene, which includes two expression cassettes, where the first cassette contains a polynucleotide which codes a first polypeptide consisting of a) a DNA-binding domain represented by an ecdysone receptor domain (EcR), GAL4 or LexA and b) a ligand-binding domain (LBD) of EcR containing at least a mutation in a position corresponding to position 132 in the ecdysone receptor sequence Chotistoneura fumiferctna (CfEcR), which increases sensitivity of the system to diacyl hydrazine ligands, and the second expression cassette contains a polynucleotide which codes a second polypeptide consisting of a) a transactivation domain represented by a transactivation domain of EcR, VP16, an acidic activator B42 or p65, and b) a second LBD which is a nuclear receptor domain capable of dimerisation with a first mutant LBD, preferably a ligand-binding domain of retinoid X receptor.
EFFECT: use of present invention enables to control expression of recombinant proteins with synthetic ligands and, consequently, obtain the desired products in any type of animal and plant host cells.
12 cl, 14 tbl, 10 ex
