METHOD FOR COLLAGEN FIBRE MATURITY EVALUATION Russian patent published in 2012 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: prepared biological material is fixed in 10% neutral formalin, imbedded in paraffin; sections are made in a microtome, thereafter placed on a slide surface and chemically purified from paraffin with using xylene with collagen fibre maturity being evaluated by two independent criteria: a degree of allowed cross striation (D-periodicity) and a collagen fibre hardness wherein the degree of allowed cross striation is evaluated as a pitch height of a slot and an overlap of separate microfibrilles by Fourier analysis (Image Analysis 3, NT-MDT) on the basis of axially transverse and longitudinal profile analysis on topographic images pre-processed by a non-linear high-frequency filter with the use of Image Analysis 3 program (NT-MDT), while the collagen fibre hardness is evaluated as a failed wave phase of a cantilever of a scanning probe microscope. The sample is tested in the air medium.

EFFECT: along with simplification of the method, higher result reliability is ensured.

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