METHOD FOR EVALUATING PATTERN OF INTERMICROBIAL INTERACTIONS Russian patent published in 2012 - IPC C12Q1/00 C12N1/20 

FIELD: medicine.

SUBSTANCE: microbial cultures are grown on LB-broth that is followed by McFarland standartisation to 0.5. The grown microbial cultures are introduced in wells of a polystyrene plate and added with LB-broth to form a monofilm or added with a broth culture to form a mixed biofilm. The plates are kept in a thermostat at 37°C for 48 hours to form biofilms. The formed biofilms are washed with distilled water and coloured with 0.1% aqueous gentian violet 200 mcl for 45 minutes in the dark, and the biofilms are thoroughly washed for three times with distilled water that is followed by drying the plate and colour intensity biofilm testing. For this purpose, the colourant is eluted in 96% alcohol; the eluate is analysed for an optical density in a spectrophotometer; this value matches to a film formation level; the average optical densities of the eluate of the mixed biofilms and the total optical densities of the eluate of the monoculture biofilms are compared, and if observing no reliable differences, the neutral pattern of interactions are stated; if the value for the mixed biofilm is less than the total value for the monofilms, the antagonistic pattern of interactions is concluded, while the value for the mixed biofilm exceeding the total values for the monofilms enables stating a synergistic pattern of the intermicrobial interaction. The total optical densities of the eluate of the monoculture biofilms shall not exceed 4.

EFFECT: invention enables evaluating the pattern of intermicrobial interaction and predicting a developing mixed infection.

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