METHOD OF DETECTING MICROORGANISMS IN SAMPLE Russian patent published in 2012 - IPC C12Q1/04 G01N33/487 C12N13/00 C12R1/19 C12M1/34 C12M1/42 

FIELD: chemistry.

SUBSTANCE: invention relates to microbiology. Biological or artificial fluid medium containing the selected microorganism is centrifuged. Supernatant fluid is filtered. A series of diluted samples corresponding to increase in filtrate dilution of up to 10^-15 is obtained. The samples are exposed to an electric, magnetic and/or electromagnetic excitation field. Electric signals detected by a solenoid and the digital record of said electric signal after passing through an analogue-to-digital converter are analysed. Diluted samples for which characteristic electric signals, whose amplitude is 1.5 times higher than that of the background noise signals emitted by water, are obtained are selected. Test tubes with equal volume of diluted samples are placed in protective jackets to protect the diluted solutions from external electromagnetic fields. The solution contained in test tube T1 is used as the standard solution. Test tube T2 is placed in the immediate proximity of the sample or is brought into contact with sample X which is presumed to contain the selected microorganism (e.g., E.coli). The obtained electromagnetic signals are compared. Suppression of the signal indicates presence of the microorganism in sample X.

EFFECT: group of inventions enables to obtain reagents and a system for detecting microorganisms in a sample, and detect infection in humans or animals.

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