METHOD OF EVALUATING ECOLOGICAL STATE OF ENVIRONMENT Russian patent published in 2012 - IPC C12Q1/02 C12N1/00 

FIELD: chemistry.

SUBSTANCE: method involves collecting samples of planktons inhabiting a water body, determining the contamination level through analysis thereof and evaluating analysis results, wherein the contamination level is determined by determining conservative and variable gene sequences of a plankton from the sample, followed by molecular physiological analysis to determine evolutional relationships of the analysed organism with other saprobionts. Analysis results are evaluated as follows: at high (more than or equal to 70%) value of bootstrap cluster support on the conservative and variable gene, including the analysed plankton and resistant saprobionts. The following conclusions are made. When the resistant indicator organisms of xeno- and/or xeno-oligosaprobic water bodies and the analysed plankton merge into a single cluster, the water body is in a safe ecological state and there is no threat of negative human impact. If the resistant indicator organisms of oligo- and/or oligo-mesosaprobic water bodies and the analysed plankton merge into a single cluster, the water body is in an unstable ecological state, is capable of self-restoration and does not need additional nature conservation activities. When the resistant indicator organisms of meso- and/or meso-polysaprobic water bodies and the analysed plankton merge into a single cluster, the water body is in a bad ecological state and needs nature conservation activities. When resistant indicator organisms of polysaprobic water bodies and the analysed plankton merge into a single cluster, there is a local ecological disaster and there is need for urgent remedial measures.

EFFECT: high reliability of biomonitoring results for use without limitation of territory, regardless of the geographical location of the analysed water body.

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