FIELD: chemistry.
SUBSTANCE: invention relates to genetic engineering and microbiological industry, as well as a genetic structure for exposing protein on the Yarrowia lipolytica yeast cell wall surface. The disclosed structure has a promoter, a terminator, a signal sequence, a selective marker, a nucleotide sequence which encodes an immobilised protein, and a nucleotide sequence which encodes an amino acid sequence containing an amino acid sequence selected from a group of sequences given in the list of sequences under number SEQ ID NO:1 or SEQ ID NO:2, or SEQ ID NO:3, or SEQ ID NO:4, or SEQ ID NO:5, or SEQ ID NO:6.
EFFECT: invention simplifies the process of purifying the protein produced by the cell and can be used in microbiological synthesis of proteins.
7 dwg, 11 ex, 1 tbl
Title | Year | Author | Number |
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YEAST STRAIN Yarrowia lipolytica - PRODUCER OF CELL-BOUND LIPASE | 2012 |
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YARROWIA LIPOLYTICA YEAST TRANSFORMANT PRODUCING LINALOOL | 2022 |
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RU2422526C2 |
RECOMBINANT PROTEIN CONSTRUCT DSD-sp-β-GAL EXHIBITING ENZYMATIC ACTIVITY OF THERMOSTABLE β-GALACTOSIDASE (LACTASE) AND AFFINE CONNECTABLE WITH DEXTRANE, PLASMID DNA pGD-10 DETERMINING DSD-sp-β-GAL BIOSYNTHESIS, AND Escherichia coli DH5α/PGD-10 PRODUCER STRAIN | 2009 |
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RU2428477C2 |
Authors
Dates
2012-05-27—Published
2010-11-13—Filed