FIELD: biochemistry.
SUBSTANCE: present invention relates to biochemistry, particularly to method of modifying LacZ gene of Escherichia coli. For implementation of method DNA fragment containing coding area of LacZ gene is obtained by means of polymerase chain reaction using oligonucleotide primers, making it possible to introduce additional GGA codon coding glycine immediately after initiating codon ATG.
EFFECT: present method enables to adapt said gene for its expression in cells Yarrowia lipolytica.
1 cl, 2 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN YARROWIA LIPOLYTICA - PRODUCER OF OBESUMBACTERIUM PROTEUS ENCAPSULATED PHYTASE | 2017 |
|
RU2664476C1 |
| RECOMBINANT STRAIN YARROWIA LIPOLYTICA - PRODUCER OF ENCAPSULATED PHYTASE PAENIBACILLUS SPECIES | 2023 |
|
RU2814490C1 |
| INTEGRATIVE pQ-SRUS GENETIC STRUCTURE FOR OBTAINING Yarrowia lipolytica YEAST STRAINS HAVING ABILITY FOR HOMOLOGICAL RECOMBINATION OF GENOME OF MITOCHONDRIA DUE TO EXPRESSION OF RecA PROTEIN GENE OF BACTERIAL ORIGIN | 2014 |
|
RU2562869C1 |
| RECOMBINANT YEAST STRAIN YARROWIA-LIPOLYTICA LIPASE PRODUCER | 2011 |
|
RU2451075C1 |
| METHOD OF HOMINAL MITOCHONDRIAL GENOME COPYING IN YARROWIA LIPOLYTICA YEAST CELLS | 2016 |
|
RU2660715C2 |
| MODIFIED NUCLEOTIDE SEQUENCE ENCODING A POLYPEPTIDE WITH THE ACTIVITY OF PHYTOENE SYNTHASE AND GERANYLGERANYL PYROPHOSPHATE SYNTHASE (VARIANTS), ITS USE IN THE CONSTRUCTION OF CAROTENOID-PRODUCING YARROWIA LIPOLYTICA YEAST TRANSFORMANTS | 2021 |
|
RU2794980C1 |
| STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING | 2006 |
|
RU2315105C1 |
| YARROWIA LIPOLYTICA YEAST TRANSFORMANT PRODUCING LINALOOL | 2022 |
|
RU2809554C1 |
| YEAST STRAIN Yarrowia lipolytica - LIPASE PRODUCER | 2007 |
|
RU2355754C1 |
| INTEGRATIVE VECTOR Random-URA3-RPT FOR SERIAL INTRODUCTION OF MULTIPLE COPIES OF GENETIC ELEMENTS IN YEAST Yarrowia lipolytica | 2006 |
|
RU2376376C2 |
