FIELD: biotechnology.
SUBSTANCE: recombinant plasmid pBU-LipA has been proposed, which ensures the synthesis of the recombinant lipase A protein of the Bacillus natto IAN strain weighing 19.3 kDa in Bacillus subtilis cells. Plasmid size is 7444 bps. The plasmid is characterized by a physical and genetic map shown in Figure 1. The recombinant gene encoding the target protein of Bacillus natto lipase A is inserted at the BamHI and AatII restriction sites into the polylinker region of the pBU vector, which contains a strong promoter and signal sequence.
EFFECT: effective production of recombinant lipase A protein and its export into the culture medium.
1 cl, 2 dwg, 1 tbl, 3 ex
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