YARROWIA LIPOLYTICA YEAST TRANSFORMANT PRODUCING LINALOOL Russian patent published in 2023 - IPC C12N1/19 C12N15/52 C12N15/81 C12P7/04 

FIELD: biotechnology.

SUBSTANCE: transformation of the yeast Y. lipolytica has been proposed that produces the monoterpenoid linalool, containing in the chromosome at least one copy of each of the following genes: Y. lipolytica HMGR1 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, ERG12 Y. lipolytica gene, encoding mevalonate kinase, modified gene ERG2^F88W-N119W Y. lipolytica, encoding farnesyl pyrophosphate synthase, codon-optimized CrGPPS Catharanthus roseus gene, encoding the enzyme geranyl pyrophosphate synthase, the nucleotide sequence of which is given in the sequence listing under SEQ ID NO: 1, and a codon-optimized LIS Actinidia arguta gene encoding linalool synthase, the nucleotide sequence of which is given in the sequence listing under SEQ ID NO: 2. A yeast strain Y. lipolytica “ВКПМ Y-5015”, which produces linalool, has also been proposed.

EFFECT: invention ensures increased production of linalool.

2 cl, 8 dwg, 1 tbl, 7 ex