FIELD: medicine.
SUBSTANCE: method involves: (a) preparing a tissue sample from a subject's wound; (b) treating said tissue sample; (c) separating glycine, proline and hydroxyproline in the treated tissue sample by high-pressure liquid chromatography to derive the analyte peak areas; (d) measuring the tissue concentrations of glycine, proline and hydroxyproline; (e) correlating the tissue concentrations of glycine, proline and hydroxyproline and total tissue collagen; and (f) comparing total tissue collagen in the wound and total tissue collagen in the intact skin to assess the wound healing process.
EFFECT: method shows accuracy, specificity and reliability even in the small amounts of collagen found in the wounds started to heal.
17 cl, 2 tbl, 3 dwg
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