FIELD: medicine.
SUBSTANCE: acne surface is pre-disinfected in the aseptic environment with 2% chlorhexidine or 96° ethanol to prepare 3-4 imprinted smears by attaching a sterile slide to a pathological centre with covering an apparently health skin area and applying a pathological acne secretion on the slide with using a tampon. The prepared imprinted smears are air dried, fixed in 96° ethanol or above an alcoholic lamp and stained in methylene blue by Romanowsky-Giemsa and Gram wherein fuchsin is replaced by neutral red. If the cytogram shows no inflammatory reaction by the 6-7th day from the beginning of treatment: observing single neutrophilic leukocytes with no destruction, fibroblasts and polyblasts (8.87% and 21.8%, respectively), the absence of microflora and the presence of epithelisation process - a plenty of epithelium cells, the absence of slime, the therapy is assessed to be effective.
EFFECT: more accurate assessment of controlling clinical effectiveness in acne that enables well-timed therapeutic correction.
2 cl, 2 tbl
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Authors
Dates
2012-06-20—Published
2011-04-27—Filed