METHOD OF DIVIDING SWINE BLOOD INTO PLASMA AND ERYTHROCYTE MASS Russian patent published in 2012 - IPC A61K35/16 A61K35/18 A61K38/42 

FIELD: medicine.

SUBSTANCE: invention relates to field of biotechnology and deals with method of dividing swine blood into plasma and erythrocyte mass. Claimed method includes mixing blood with anticoagulant during its sampling from animal. As anticoagulant, two-component solution is used: 4% solution of sodium citrate (Na₂C₆H₅O₇) with addition of citric acid until pH from 5.0 to 6.0 is achieved, it is mixed with blood in ratio 1:10 or 0.75% solution of sodium phosphate disubstituted (Na₂HPO₄) with addition of citric acid until pH from 5.0 to 5.5 is achieved, its is mixed with blood with ratio 1:4. Blood mixed with anticoagulant is cooled to temperature 4°C, centrifuged with frequency of rotation 4000÷5000 rev/min and process duration 5÷15 min.

EFFECT: claimed invention makes it possible to preserve nativity and prevent undesirable hemolysis of erythrocytes which results in increase of purity of obtained blood fractions.

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