METHOD FOR DETECTION OF ACTION OF VAGINAL EPITHELIOCYTES ON BIOLOGICAL BACTERIAL PROPERTIES (VERSIONS) Russian patent published in 2012 - IPC C12Q1/00 C12Q1/04 

FIELD: medicine.

SUBSTANCE: according to the first version, vaginal epitheliocytes are produced, separated from accompanying microflora, incubated in the Hanks' medium with added cefuroxime and lisocyme; a supernatant is separated. A microbial suspension is prepared from the daily culture of the analysed bacteria and mixed with the epithelial supernatant. It is combined with preparing a control suspension of the analysed bacteria and an epitheliocyte cultivation medium, incubated; the biological properties are measured in the bacteria of the test and control samples. A degree of the varying biological properties in the test samples is evaluated by the relation to the control sample, and the degree enables stating stimulation, indifferent or supression action of epitheliocyte on the biological bacterial properties. According to the second version - the vaginal epitheliocytes are produced, inactivated by formalin, washed out to prepare an epytheliocyte suspension in physiologic saline. A microbial suspension is prepared from the daily culture of the analysed bacteria and mixed with the inactivated epithelial suspension. It is combined with preparing a control sample from the analysed bacterial suspension and physiologic saline; the test and control samples are incubated; the biological properties are measured in the bacteria of the test and control samples; a degree of the varying biological properties in the test samples is evaluated by the relation to the control sample, and the degree enables stating stimulation, indifferent or suppression action of epitheliocyte on the biological bacterial properties.

EFFECT: enabled detection of an ability of the vaginal epitheliocyte to regulate the biological bacterial properties of various taxons enabled by stimulation, inhibition and indifferent action of the epithelial supernatants or the contact epithelial bacterial action on the biological bacterial properties that enables extended presentations of formation and maintenance of human body microbiocenosis; the derived knowledges are used to develop methodological approaches to creating new methods and preparations for correction of dysbiotic states of female reproductive tract.

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