FIELD: genetic engineering; microbiology; virology.
SUBSTANCE: recombinant strain of Escherichia coli JM 109 2-428 is proposed for use as a positive control in evaluating the efficiency of cDNA amplification of the West Nile virus 2 genotype. The strain is a producer of the recombinant pWNV2protC plasmid, which carries a fragment of the West Nile virus 2 genotype genome with a size of 428 b.p., which is a sequence of 5'-untranslated area of the genome, and a section of the West Nile virus polyprotein gene encoding the capsid protein. The strain is deposited in the State Collection of Pathogenic Bacteria of the Federal Public Health Institution Russian Anti-Plague Research Institute "Microbe" of Russian Federal State Agency for Health and Consumer Rights, under the number KM 2050.
EFFECT: invention provides efficient replication and separation of the recombinant pWNV1protC plasmid with a high yield.
1 cl, 2 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| ESCHERICHIA COLI JM 109 1-430 BACTERIAL STRAIN - PRODUCER OF THE RECOMBINANT PWNV1PROTC PLASMID, WHICH CARRIES THE SEQUENCE OF THE 5'-UNTRANSLATED AREA AND A SECTION OF THE WEST NILE VIRUS 1 POLYPROTEIN GENE OF THE GENOTYPE ENCODING THE CAPSID PROTEIN | 2020 |
|
RU2744095C1 |
| BACTERIAL STRAIN ESCHERICHIA COLI JM 109 4-162, PRODUCER OF RECOMBINANT PLASMID PWNV4PROTC, CARRYING SEQUENCE OF POLYPROTEIN GENE SEGMENT OF WEST NILE VIRUS GENOTYPE 4 ENCODING CAPSID PROTEIN | 2020 |
|
RU2739333C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING WEST NILE VIRUS LINEAGE 4 | 2020 |
|
RU2737396C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS GENOTYPE 2 | 2019 |
|
RU2715617C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS 1 GENOTYPE | 2019 |
|
RU2715625C1 |
| RECOMBINANT PLASMID VECTOR pET32-WNV-DIII, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT DOMAIN III OF STRUCTURAL GLYCOPROTEIN E OF WEST NILE VIRUS IN ESCHERICHIA COLI CELLS, ESCHERICHIA COLI BL21(DE3)-DIII-WNV CELL LINE STRAIN AND DIII-WNV RECOMBINANT PROTEIN FOR PREPARING IMMUNOBIOLOGICAL PREPARATIONS | 2024 |
|
RU2821341C1 |
| RECOMBINANT STRAIN OF Escherichia coli TG1(pRVMoscow3253G-L) FOR OBTAINING SET OF PCR-STANDARDS AND SET OF PCR-STANDARDS FOR DETERMINATION OF CONCENTRATION OF RABIES VIRUS "Moskva 3253" IN RABIES ANTIGEN | 2012 |
|
RU2511029C2 |
| GENETIC CONSTRUCT FOR GENE EXPRESSION mNG_CD4-CXCR4, RECOMBINANT PLASMID rVSV_MNG_CD4-CXCR4 AND A RECOMBINANT VESICULAR STOMATITIS VIRUS STRAIN rVSV_mNG_CD4-CXCR4, WHICH PROVIDES TARGETED VIROLYSIS OF CELLS EXPOSING HIV-1 GP120/GP41 PROTEINS TROPISM X4 ON THEIR SURFACE | 2021 |
|
RU2768032C1 |
| GENETIC CONSTRUCT FOR EXPRESSION OF mNG_CD4-CCR5 GENES, RECOMBINANT PLASMID rVSV_mNG_CD4-CCR5 AND RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS rVSV_mNG_CD4-CCR5 PROVIDING TARGETED VIROLYSIS OF CELLS DISPLAYING HIV-1 PROTEINS GP120/GP41 OF R5 TROPISM ON THEIR SURFACE | 2021 |
|
RU2769125C1 |
| IMMUNOBIOLOGICAL AGENT FOR THE PREVENTION OF DISEASES CAUSED BY TICK-BORNE ENCEPHALITIS VIRUS BASED ON A RECOMBINANT VIRUS OF THE GENUS FLAVIVIRUS | 2022 |
|
RU2795800C1 |
