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2 608 505

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IPC

C12N1/00(2006-01-01)

C12Q1/68(2006-01-01)

(21) (22)

Application

2016103360, 2016-02-02

(24)

Start date

2016-02-02

(22)

Actual filing date

2016-02-02

(45)

Published

2017-01-18

(72)

Inventor

Viktorov Dmitrij ViktorovichZakharova Irina BorisovnaKuzyutina Yuliya AleksandrovnaLopastejskaya Yana Anatolevna

(73)

Holder

Federalnoe Kazennoe Uchrezhdenie Zdravookhraneniya Nauchno-Issledovatelskij Protivochumnyj Institut" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka

SET OF 5'-PHOSPHORYLATED OLIGONUCLEOTIDE PRIMERS FOR AMPLIFICATION BY POLYMERASE CHAIN REACTION OF COMPLETE CODING SEQUENCE OF OMPA/MOTB OF BURKHOLDERIA PSEUDOMALLEI Russian patent published in 2017 - IPC C12N1/00 C12Q1/68

Abstract RU 2608505 C1

FIELD: biology.

SUBSTANCE: invention relates to molecular biology and biotechnology. Invention represents set of 5'-phosphorylated oligonucleotide primers for amplification by polymerase chain reaction of gene ompA/motB, coding surface protein OmpA/MotB of causative agent of melioidosis.

EFFECT: invention allows to produce high concentration of DNA fragment, carrying full sequence of ompA/motB, coding highly immunogenic surface protein OmpA/MotB of causative agent of melioidosis, which allows to perform in future its cloning in expressing vector for purpose of accumulation of recombinant antigen.

1 cl, 1 dwg, 2 tbl, 2 ex

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RU 2 608 505 C1

Authors

Viktorov Dmitrij Viktorovich

Zakharova Irina Borisovna

Kuzyutina Yuliya Aleksandrovna

Lopastejskaya Yana Anatolevna

Dates

2017-01-18—Published

2016-02-02—Filed