FIELD: medicine.
SUBSTANCE: substratum o-dianisidine is introduced in blood plasma diluted in phosphate-citrate buffer (pH 5.5). After hydrogen peroxide added in the concentration of 2 mM, the substratum oxidation rate is evaluated by sepectrophotometry in the presence of the myeloperoxidase inhibitor - 4-aminobenzoic acid hydrazide. Peroxidase activity of blood plasma haemoglobin is described as the substratum o-dianisidine oxidation rate.
EFFECT: method enables fast and reliable evaluation of haemoglobin contribution to total peroxidase activity of blood plasma that is applicable in prediction of the developing diseases and choosing a therapeutic approach.
2 dwg, 1 tbl, 3 ex
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