METHOD FOR DETERMINING FUNCTIONAL STATE OF BLOOD PLASMA MYELOPEROXIDASE Russian patent published in 2012 - IPC G01N33/68 

FIELD: medicine.

SUBSTANCE: method involves introducing a substrate of o-dianisidine into blood plasma dissolved in a buffer solution, spectrophotometry of oxidation rate after adding hydrogen peroxide and determination of peroxidase activity of myeloperoxidase by a difference of o-dianisidine oxidation rates in the absence and in the presence of 4-aminobenzoic acid hydrazide. Additionally, the concentration of blood plasma myeloperoxidase is determined by introducing rat's affine myeloperoxidase antibodies into plate wells followed by adding plasma samples and myeloperoxidase standard. It is added with rabbit's myeloperoxidase antibodies and horseradish peroxide-conjugated rabbit's IgG antibodies. Then a myeloperoxidase specific activation coefficient C_sa is calculated as a relation of enzyme activity to its concentration. If the values C_sa is more than 1.65 optical density units/pg/min, the disturbed functional state of myeloperoxidase is stated.

EFFECT: invention provides fast and reliable determination of the functional state of blood plasma myeloperoxidase that enables predicting developing a number of diseases, improving diagnoses and taking well-timed actions aiming at enzyme strength control.

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