FIELD: chemistry.
SUBSTANCE: chicken egg albumin is mixed with an equal volume of a mixture 0.5 M aqueous trichloroacetic acid and an organic solvent in volume ratio 1:1.8-2.3. The formed residue is separated by filtering at 0-5°C. The filtrate is then mixed with an organic solvent in volume ratio of the organic solvent to the filtrate of 3-3.5:1. The supernatant fluid is then removed by decantation at 0-5°C. Low-molecular impurities are removed from ovomucoid through ultrafiltration. The ovomucoid is lyophilically dried. The organic solvent used is a mixture of ethanol and acetone in volume ratio 40-65:60-35.
EFFECT: high output of ovomucoid and high degree of purity.
1 tbl, 5 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PRODUCING OVOMUCOID | 0 |
|
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Authors
Dates
2012-09-10—Published
2011-05-12—Filed