CHROMATOGRAPHIC METHODS Russian patent published in 2012 - IPC B01D15/36 B01D15/20 B01J49/00 C07K1/18 

FIELD: medicine.

SUBSTANCE: invention relates to chromatographic purification of polypeptides. Disclosed is a method of producing monopegylated erythropoietin, involving chromatographic purification thereof and regeneration of the cation-exchange chromatographic column after elution of the monopegylated erythropoietin. Elution of the adsorbed monopegylated erythropoietin from the column is carried out with a buffered aqueous solution containing sodium chloride in concentration of at least 500 mM. The column is then washed with purified water, 0.5 M sodium hydroxide solution is then fed into the column and the column is rewashed. A solution containing 0.5 M sodium dihydrophosphate and 1 M phosphoric acid is fed into the column and the column is washed. 0.5 M sodium hydroxide solution is fed into the column for at least 4 hours and the column is finally regenerated by washing it with purified water.

EFFECT: invention provides high purity of monopegylated erythropoietin.

10 cl, 2 dwg, 1 tbl, 1 ex