IMPROVED METHOD OF PEGYLATING PROTEINS Russian patent published in 2013 - IPC C07K14/535 A61K47/48 

Abstract RU 2472806 C1

FIELD: chemistry.

SUBSTANCE: method involves conjugation of r-metHuG-CSF with PEG-aldehyde at the free amino group at the N-end of r-metHuG-CSF in the presence of a reducing agent in a buffer solution for pegylation, which contains a polyol of formula CnH2n+2On, where n equals 3-6, or carbohydrate or derivative thereof, where concentration of said polyol or carbohydrate or derivative thereof is in the range of 0.1%-10%, wt/wt.

EFFECT: high output of the r-metHuG-CSF pegylation reaction.

15 cl, 12 dwg, 1 tbl, 10 ex

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RU 2 472 806 C1

Authors

Mokhe Nikkhil Umesh

Tarur Radkhakrishnan Venkatasubramaniam

Palival Dinesh Kumar

Saksena Divija Lal

Patil Nilesh Dagdu

Chandrakesan Muralidkharan

Paval Digamber Sripati

Shekkhavat Rakesh

Kkhare Aruna

Zavar Sagar Satianarajan

Mal'Pure Pankadzh Prabkhakar

Rana Dilip Kumar

Dates

2013-01-20Published

2009-05-04Filed