FIELD: chemistry.
SUBSTANCE: method involves conjugation of r-metHuG-CSF with PEG-aldehyde at the free amino group at the N-end of r-metHuG-CSF in the presence of a reducing agent in a buffer solution for pegylation, which contains a polyol of formula CnH2n+2On, where n equals 3-6, or carbohydrate or derivative thereof, where concentration of said polyol or carbohydrate or derivative thereof is in the range of 0.1%-10%, wt/wt.
EFFECT: high output of the r-metHuG-CSF pegylation reaction.
15 cl, 12 dwg, 1 tbl, 10 ex
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