FIELD: chemistry.
SUBSTANCE: present invention relates to a method of purifying mono-pegylated erythropoietin using two cation-exchange chromatography steps, where the same type of cationite is used at both cation-exchange chromatography steps.
EFFECT: method of producing mono-pegylated erythropoietin in a substantially homogeneous form.
17 cl, 3 dwg, 2 tbl, 3 ex
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