FIELD: chemistry.
SUBSTANCE: invention relates to medicine and a method of purifying a protein containing erythropoietin and one polyethylene glycol residue from reaction by-products or from unreacted raw material using cation-exchange chromatography. The method employs chromatographic material SP Sephacryl S 500 HR, which is balanced by a solution with conductance of 21 mS/cm and linear gradient elution.
EFFECT: method enables to purify a protein in one step with high purity and output.
9 cl, 7 dwg, 5 tbl, 4 ex
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