FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to the preparation of conjugates of a physiologically active polypeptide with a non-peptidyl polymer and a constant region of an immunoglobulin, and can be used in medicine to create long-acting compositions of physiologically active polypeptides that improve adherence to medication.
EFFECT: method differs by varying the concentrations of the reducing agent from 1 mM to less than 20 mM at the first stage and from 1 to 100 mM at the second stage of reductive amination, thereby overcoming the traditional problems of low yield of a modified polypeptide with a prolonged action profile.
25 cl, 4 tbl, 7 ex
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