LIFE-TIME DIAGNOSTIC TECHNIQUE FOR Ascaris lumbricoides AND Ascaris suum BY EGG MICROSTRUCTURE Russian patent published in 2012 - IPC G01N33/48 A61D99/00 

FIELD: medicine.

SUBSTANCE: life-time differential diagnostic technique for Ascaris lumbricoides and Ascaris suum involves ascarid egg sampling on a slide, clearing in the mixture of 98.5% glycerol of density 1.256 and 40% lactic acid taken in proportions 1:1 then the eggs are covered with a cover slip and examined under the microscope at magnifications: 600 and 900 under immersion; for the purpose of diagnosing, the microstructural differences of eggs are determined, and ascarid types are differentiated by the following signs: Ascaris lumbricoides in the 1-st version: has a stylette on one pole; and on the other one: a cone-shaped metallic cap embraced by microstructure ends extending from the 2-nd shell from the opposite pole; the 2-nd shell: on one side - erased pimples; on the other side - flat, leaflike ones; in the 2-nd version: the cap is brush-shaped with its thin leg connected to an embryo through a gap drawn apart and a lifter hook-like gate of the 2-nd shell, in a basal portion - yellowish-white: a tooth-like plate; on the other one: a hole is open on the pole; the 3-rd shell functions as a plug; has 3 pimples; the 2-nd shell: 2 coupled ovals, fringed borders and grooved pimples; Ascaris suum the 1-st version: has the cap with 2 pimples on an apex on one of the poles and 3 tooth-like projections in the basal portion; on the other one: a slightly pointed pole; the 2-nd version: the 2-nd shell, the embryo: an evident pimpling; one pole is densely covered with large pimples; on the other one, the stylette with the microstructure in the form of a predatory animal head coupled with the 2-nd dense shell.

EFFECT: technique provides life-time ascarid differentiation by egg microstructure to type: Ascaris lumbricoides and Ascaris suum.

1 ex, 8 dwg