METHOD FOR DETECTION OF HELMINTHES, TICK EGGS AND PROTOZOAN OOCYSTS IN SOIL SAMPLES Russian patent published in 2012 - IPC G01N33/24 

FIELD: medicine.

SUBSTANCE: method for detection of helminthes, tick eggs and protozoan oocysts in soil samples consisting in the fact that a soil sample 25 g is placed in a centrifugal test-tube 250 ml and filled with water 150 ml; the mixture is thoroughly mixed with a glass rod for 5 min or with an electrical mixer for 1 min and centrifuged for 3 min at 800-1000 rpm; water is poured off, while the test tubes are added with a three-component flotation system 150 ml containing saturated solutions of zinc chloride (2 kg of ZnCl₂ or 1 l), sodium chloride (0.42 kg of NaCl per 1 l) and glycerol taken in proportions 1:1:1; the test tube is added with the same solution to form a convex meniscus, covered with a degreased glass so that it touches a fluid layer; 20-30 min. later, the glass is removed and observed under microscope; the preparation is recommended to be drawn for 2-3 times. The detectability of protozoan oocysts and tick eggs increases by 76.6 and 87.1% respectively.

EFFECT: method enables considerably increased a percentage of detected eggs of such soil-transmitted helminthes as human and swine ascarid, twice reducing time for expecting the analysis results.

4 tbl, 2 ex