FIELD: medicine.
SUBSTANCE: invention refers to veterinary science. A technique consists in whipworm egg sampling on a slide plate, clarification in mixed 98.5% glycerine of density 1.256 and 40% lactic acids in the ratio 1:1; then the eggs are covered with a cover glass and microscopically examined at magnification of 600 and 900 under immersion. The whipworm types are differentiated by the following signs: Trichocephalus ovis has wide plug caps, a gentle, thin, transparent 1st outer coat which covers the whole egg and it is clearly visible close to the poles only at magnification of 900, a smooth surface of a 2nd outer keratinised coat; the plugs are short, without a marked neck at the poles; the plugs do not couple with an embryo, and slightly project beyond the limits of the egg coats; the embryo packs the whole internal space of the eggs, or is ellipsoid and located in the middle of the egg; the base collars are thickened and finger-shaped at the top; the embryo mass is large-grained with green granules; the plug necks are spiral and consist of 5 rings, the third in succession of which from an apical end is twice wider than the others. Trichocephalus skrjabini has short caps at the egg poles, evident uneven surfaces of the 2nd outer keratinised egg coats; the embryo represents a yellow-brown granular mass and is curved at the poles; the plug base collars are thick; the plug necks are spiral and consist of 6 rings; Trichocephalus capreoli has long plugs strongly projecting over the egg surface and short caps, a thick keratinised surface of the 2nd outer egg coat with weak porosity; the plug base collars are thick; the embryo is curbed at the poles and represents a close-grained mass; the plug necks are spiral and consist of 8 rings.
EFFECT: technique allows for lifetime type differentiation of whipworms by the egg microstructure.
3 dwg, 1 ex
