FIELD: veterinary medicine.
SUBSTANCE: method includes taking eggs of Trichocephalus and Thominx on a glass slide, clearing them in a mixture of 98.5% glycerol solution with the density of 1.256 and 40% lactic acid taken in the ratio of 1:1. Then the eggs are covered with a coverslip and examined under a microscope at magnifications of 600, 1200 and 900 under immersion. Trichocephalus vulpis has a long with a marked neck and broad hats and corks protruding over the surface of the shells at the eggs poles, delicate, thin, transparent 1st outer shell that covers the whole egg and is visible near the poles in the form of crystalline microstructures; the eggs have: the spikes on the 2d shell and stilettos at the poles; the 3d shell is visible in the area of the corks in the form of a semi translucent triangular microstructure, passing from the eggs to the liquid space of the 1st shell; the necks of the corks are of helical shape with 3 rings; the 3d ring is convex and 2 times wider than the others, it holds the neck base of the corks with the inner shell of the embryo, Thominx (Capillaria) aerophilus has asymmetric eggs and short opaque corks at the eggs poles; the 1st shell surrounds the whole egg and it is visible at the poles and in the area of the corks; the surface of the 2nd outer keratinised shell of the eggs is fibrous and torulose; the corks consist of: a hat, 2 rounded processes located as the triangle under it and a lower (3rd) radial row consisting of five processes; all the elements of the cork are of white-yellow colour.
EFFECT: method enables to differentiate in vivo Trichocephalus vulpis and Thominx aerophilus from domestic and wild predatory to species on the microstructure of eggs.
8 dwg, 1 ex
