METHOD FOR QUANTITATIVE ASSESSMENT OF ASSIMILATED DRUG Russian patent published in 2012 - IPC G01N33/52 

FIELD: medicine.

SUBSTANCE: what is described is a method for the quantitative assessment of an assimilated drug involving the accompanying uptake of a visualising substance, and the studies conducted over a certain period of time wherein the visualising substance is presented by a fluorescent food additive mixed with the main drug in constant weight proportions F:X=φ:(1-φ) wherein F is a dose of the fluorescent food additive mg/kg of weight a day, X is a dose of the drug mg/kg of weight a day prescribed by a doctor, φ is a weight fraction of the fluorescent food additive while the studies are conducted with the use of spectrophotometry before and 12-72 h after the uptake of the mixture with the specified epithelial areas being exposed to laser of wave length 532 nm, continuous power 5÷10 mWt; fluorescence intensity is recorded within the range of wave lengths 650-690 nm. - J₀(λ) and J₁(λ) respectively, while the amount of the drug is described by formula: wherein C_J(S) is a dependence functional of fluorescence signal integral of the used visualising substance within its characteristic range of wave lengths found by the calibration graph for chlorophyll pigment as the fluorescent food additive wherein the value S_J calculated by formula: wherein λ is wave length, nm, while J₀(λ). and J₁(λ). are the fluorescence power values (in the identical standard optical units) measured before the studies and during a period of reference time t (hour) respectively.

EFFECT: method is simple, applicable, provides non-invasive instant control in vivo of the dosage of the assimilated pharmaceutical preparation.

1 ex, 2 dwg