METHOD FOR DETERMINING SPECIFIC CELL IMMUNE RESPONSE TO MEASLES AND RUBELLA VIRUS ANTIGENS Russian patent published in 2012 - IPC G01N33/533 G01N33/62 

FIELD: medicine.

SUBSTANCE: method under the invention provides incubation of a suspension of mononuclears recovered from blood, monensin 10 mcM, fluorochrome-marked CD 107a monoclonal antibodies and cytotoxic T-lymphocyte degranulation inductors - measles and rubella viruses in the complete culture medium RPMI-1640 with added 2mM L-glutamine, gentamycin and 10% embryo calf serum for 15 hours at 37°C in the 5% CO₂ environment and 100% humidity. The incubation is followed by T-lymphocyte colouring by fluorochrome-marked CD8 antibodies and recording of a subpopulation of T-lymphocytes expressing CD8^high and CD107a in a flow cytofluorometry. The quantitative evaluation of the T-lymphocytes to be found is expressed in percent as compared with the reference (without degranulation inductors).

EFFECT: method under the invention is simple and practical, and enables stating both infectious, both vaccine immunity against measles and rubella viruses.

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