FIELD: medicine.
SUBSTANCE: proposed group of inventions refers to medicine, particularly immunology. Disclosed is a method for verifying the immunogenicity of vaccine antigens in vitro for producing highly effective vaccines against dangerous infections, involving preparing a group of cell cultures. Each test tube is added with antibodies against CD3, CD4, CD8, then proliferating CD3 + CD4 + and CD3 + CD8 + T lymphocytes are determined, then intensity of lymphocyte proliferation is analysed among these groups of cells. From analysed antigens one is selected, which does not suppress proliferation of T effector cells in presence of T regulatory cells. What is presented is a method for increasing the immunogenicity of a vaccine, which consists in the fact that the vaccine antigen is first checked by the method described above, then it is modified by reducing the number of epitopes to the T regulatory cells, the modified vaccine antigen is synthesized and checked for the second time.
EFFECT: presented group of inventions provides higher immunogenicity of the vaccine and antigen.
3 cl, 2 dwg, 2 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYEPITOPE ANTI-TUMOUR VACCINE STRUCTURE CONTAINING EPITOPES OF TUMOR-ASSOCIATED ANTIGENS, PHARMACEUTICAL COMPOSITION AND ITS APPLICATION FOR STIMULATING SPECIFIC ANTI-TUMOR IMMUNE RESPONSE | 2016 |
|
RU2684235C2 |
| PREVENTIVE OR THERAPEUTIC POLYEPITOPIC ANTI-TUBERCULOSIS VACCINE CONSTRUCTION PROVIDING INDUCTION OF CELLULAR IMMUNE RESPONSE OF CD4+ OR CD8+ T-LYMPHOCYTES | 2011 |
|
RU2539035C2 |
| METHOD FOR PRODUCTION OF IN VITRO POPULATIONS OF ACTIVATED ANTIGENSPECIFIC ANTITUMOR-TUMOR CYTOTOXIC T-LYMPHOCYTES SPECIFIC TO TUMOR-ASSOCIATED ANTIGEN EPITOPES | 2016 |
|
RU2619186C1 |
| HIV VACCINE BASED ON MAXIMISED Gag AND Nef TARGETING ON DENDRITE CELLS | 2009 |
|
RU2539765C2 |
| POLYEPITOPE IMMUNOGENIC POLYPEPTIDES AND METHODS OF APPLICATION THEREOF | 2013 |
|
RU2598265C2 |
| VACCINE BASED ON B-CELLS, LOADED WITH LIGANDS OF T-CELLS-NATURAL KILLERS AND ANTIGEN | 2006 |
|
RU2420311C2 |
| METHOD FOR GENERATION OF SPECIFIC IMMUNE RESPONSE ON HUMAN IMMUNODEFICIENCY VIRUS ANTIGENS | 2011 |
|
RU2465324C1 |
| METHOD OF PRODUCING ANTIGEN-SPECIFIC CYTOTOXIC CELLS HAVING ACTIVITY AGAINST BREAST CANCER CELLS | 2013 |
|
RU2596920C2 |
| COMBINATION PREPARATION FOR INCREASING POTENCY OF VACCINE (VERSIONS) | 2008 |
|
RU2491090C2 |
| IMMUNOPROPHYLACTIC CANCER VACCINE | 2004 |
|
RU2556128C2 |
