METHOD FOR MAKING RESORBED POLYLACTIDE MATRIX FOR CELL CULTURE AND IMPLANTATION FOR WOUND HEALING Russian patent published in 2012 - IPC A61K31/70 A61K35/36 A61P17/02 

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and medicine and represents a method for making a resorbed polylactide matrix for cell culture and implantation for wound healing that involves preparing a resorbed hydrophilic porous polylactide matrix uniformly coated with type 1 microfibrillar collagen; for this purpose the hydrophilic porous polylactide matrix 13 mcm tp 15 mcm thick with the pore diameter of 2 mcm to 3 mcm is prepared and then coated with 0.01% type 1 collagen in 0.1% acetic acid, incubated for 30 minutes at room temperature; thereafter, the matrix surface is washed in phosphate-buffered saline pH 7.4 to remove protein not bound with a substrate; the procedure of coating with the type 1 collagen solution is performed for two more times; the hydrophilic surface of the porous polylactide matrix uniformly coated with microfibrillar collagen structures of the diameter of 10 nm to 20 nm is applicable for human keratinocyte culture.

EFFECT: invention provides more effective structure of the protein layer of the matrix, simplified and cheaper method, and reduced resorption time of the polylactide matrix.

4 cl, 3 ex, 6 dwg