FIELD: biotechnology, medicine. SUBSTANCE: three-dimensional bioactive carrier for cultivation of mammalian cells and tissues has foamy elastic polyurea-polyester polyurethane (pore size is 100-500 microns) as a polymeric base or polyvinylformal (pore size is 100-300 microns). Carrier is covered by monolayer of primary stromal cells grown on it. Cells are treated with solution containing sodium deoxycholate, sodium chloride, EDTA, phenylmethylsulfonylfluoride, ethylmaleimide, tris-HCl for intercellular matrix formation. EFFECT: enhanced quality of carrier.
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