HYALURONIDASE AND METHOD FOR USING IT Russian patent published in 2013 - IPC C12N9/26 C12N9/88 A61K38/02 A61K38/43 A61K38/51 A61P41/00 

FIELD: medicine.

SUBSTANCE: there are presented hyaluronidase protein of molecular weight approximately 44±1 kDa containing an amino acid sequence at least by 90% identical to an amino acid sequence containing SEQ ID NO: I, SEQ ID NO: 2 and SEQ ID NO: 4 presented in the description, as well as DNA coding it. What is described is a pharmaceutical medical composition for increasing tissue penetration and decreasing connective tissue viscosity containing an effective amount of recovered said hyaluronidase and a pharmaceutically acceptable excipient, a carrier, a solvent and an additive agent. The following methods are presented: 1) prevented or minimised cicatrisation involving the local introduction of said pharmaceutical composition; 2) reduced wrinkle formation by decreasing connective tissue viscosity involving the local introduction of said pharmaceutical composition; 3) relieved discomfort and pain caused by rheumatic arthritis, systemic sclerosis, peritendinitis or tendovaginitis involving the introduction of said pharmaceutical composition into a patient; 4) improved agent penetration into tissue involving the introduction of the agent together with the pharmaceutical composition into an individual.

EFFECT: invention provides extending the range of pharmaceutical preparations.

14 cl, 23 tbl, 15 dwg, 12 ex