ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS COMPLEX IMMUNODETECTION Russian patent published in 2013 - IPC G01N33/569 G01N33/533 G01N33/543 

FIELD: medicine.

SUBSTANCE: group of inventions refers to methods for specific detection of an antigen specific for the Mycobacterium tuberculosis complex of a secretory protein MPT64 in a biological sample. What is produced is an antibody recognizing an epitope MPB64 localised in any of amino acid sequences SEQ ID NO:2-4, particularly a monoclonal antibody. Thereby, there are presented immunoassay with the use of the antibody, particularly an immunoassay sandwich with the use of a first and second MPB64 antibody, immunochromatographic assay and an immunochromatographic test strip. A biological sample may be subjected to the immunoassay without cultivation or after cultivation for a period of time before the bacteria in the Mycobacterium tuberculosis complex in the sample start growing substantially. The biological sample may be pre-treated by treatment for Mycobacterium tuberculosis inactivation by dispersion or solubilisation.

EFFECT: group of inventions provides fast and safe high-accuracy diagnosis of Mycobacterium tuberculosis infection.

22 cl, 10 ex, 5 tbl, 2 dwg