FIELD: medicine.
SUBSTANCE: invention refers to medicine and concerns a method for carrying out an immunochromatographic assay with a dissociating fluorescent tag, wherein complexes comprising molecules of an antigen or antigens, specific antibodies and tag molecules are formed on a membrane test strip. After the assay is completed, the tag is extracted from the working membrane of the test strip, and a fluorescent signal is measured in a liquid volume.
EFFECT: method according to the invention provides creating the optimum conditions for tag fluorescence, as well as eliminating the harmful influence of the membrane which is impermeable to decrease an intensity of both exciting and emitted light.
1 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS COMPLEX IMMUNODETECTION | 2008 |
|
RU2473095C2 |
| METHOD OF MULTIANALYTIC IMMUNE ASSAY WITH USING MICROPARTICLES | 2008 |
|
RU2379691C1 |
| METHOD OF IMMUNOCHROMATOGRAPHIC DETERMINATION OF SPECIFIC ANTIBODIES | 2013 |
|
RU2545909C2 |
| METHOD OF IMMUNOCHROMATOGRAPHIC SERODIAGNOGY WITH SEQUENTIAL ADDITION OF REAGENTS | 2020 |
|
RU2741199C1 |
| METHOD OF CARRYING OUT IMMUNOCHROMATOGRAPHIC ANALYSIS FOR SERODIAGNOSTICS | 2012 |
|
RU2532352C2 |
| METHOD FOR SPECIFIC SUBSTANCE DETECTION IN MILK | 2012 |
|
RU2617400C2 |
| METHOD TO REDUCE LIMIT OF DETECTION OF IMMUNOCHROMATOGRAPHIC METHODS TO CONTROL CONTENT OF LOW-MOLECULAR COMPOUNDS | 2011 |
|
RU2497126C2 |
| METHOD FOR INCREASING SENSITIVITY OF IMMUNOCHROMATOGRAPHIC SERODIAGNOSTIC ASSAY USING TWO MARKERS | 2019 |
|
RU2739752C1 |
| METHOD OF DETERMINING LIGAND CONTENT IN SAMPLE (VERSIONS) | 2012 |
|
RU2517161C2 |
| IMMUNOCHROMATOGRAPHY METHOD FOR DETECTION OF ESCHERICHIA COLI IN WATER | 2005 |
|
RU2300768C1 |
