METHOD OF PRODUCING PRIMORDIAL GERM CELLS OF BIRDS Russian patent published in 2025 - IPC C12N5/07 C12N5/10 C12N5/16 A01K67/00 

FIELD: living systems technologies.

SUBSTANCE: invention relates to living systems technologies, in particular to a group of cell technologies for obtaining donor embryonic primordial germ cells of birds. Method includes selection and incubation of birds eggs to obtain a bird embryo at stage 13-14 according to Hamburger-Hamilton; round cutting of poultry egg shell with diameter of 10-15 mm is carried out. All manipulations are carried out inside the egg under the unopened outer membrane without transferring the egg contents into a specialized cuvette, through the hole in the egg shell under the unopened outer membrane with a microinjector with a glass microneedle from the dorsal aorta of the bird embryo. A blood sample is taken in volume of 5 mcl for cultivation, and when collecting a blood sample from the dorsal aorta of the embryo, the needle is inserted parallel to the artery, against the direction of blood flow. Blood samples taken from the embryo are introduced into prepared test tubes with a culture medium and stored in a heating cabinet at temperature of 37 °C until transfer to the culture plate. Primordial germ cells are cultured in a well plate with a culture medium with cell growth factors at temperature of 37 °C for 21 days.

EFFECT: increasing the number of obtained primordial germ cells from one embryo.

2 cl, 1 dwg