FIELD: biotechnologies.
SUBSTANCE: method to produce polypeptide of factor VIII provides for cultivation of cells of mammals, which express polypeptide of factor VIII, under conditions, suitable for expression of the specified popypeptide of factor VIII, which include a cell cultural medium with O-phospho-L-serine (OPLS) and extraction of the expressed polypeptide of factor VIII from cells of mammals with the help of suitable means.
EFFECT: invention makes it possible to produce popypeptide of factor VIII with increased specific activity.
11 cl, 3 dwg, 4 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CELL CULTURE WITHOUT COMPONENTS OF ANIMAL ORIGIN | 2004 |
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RU2369634C2 |
| METHOD FOR INCREASING SPECIFIC RATE AND PRODUCING IN EUKARYOTIC CELLS | 2015 |
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| MEDIUM FOR CULTIVATION OF CELLS WITHOUT PROTEINS AND SERUM | 2009 |
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| MEDIUM THAT DOES NOT CONTAIN PROTEINS AND SERUMS, AND METHOD OF MAMMAL CELL CULTIVATION IN SUCH MEDIUM | 2000 |
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RU2380412C2 |
| MEDIUM FOR CELLS CULTIVATION THAT DOES NOT CONTAIN ANIMAL PROTEINS | 2005 |
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RU2383616C2 |
| CELL CULTIVATION MEDIUM WITHOUT PROTEINS AND SERUM | 2000 |
|
RU2266325C2 |
| EXPRESSION GENETIC MAKER pOptivec/F8BDD CODING HUMAN RECOMBINANT BLOOD COAGULABILITY FACTOR VIII WITH DELETION OF B-DOMAIN AND CELL LINE DG-OV-F8BDD-18 PRODUCING HUMAN RECOMBINANT BLOOD COAGULABILITY FACTOR VIII WITH DELETION OF B-DOMAIN | 2009 |
|
RU2429294C2 |
| MEDIUM FOR CELL CULTURE THAT DOES NOT CONTAIN OLIGOPEPTIDES | 2017 |
|
RU2758802C2 |
| OLIGOPEPTIDE-FREE CELL CULTURE MEDIA | 2013 |
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RU2642269C9 |
| METHOD OF PROTEIN EXPRESSION | 2007 |
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RU2486236C2 |
