FIELD: biotechnology.
SUBSTANCE: invention relates to use of meta-tyrosine to increase specific production, which is the amount of produced polypeptide a day, Chinese hamster ovary CHO-K1 cells, which produces an exogenous polypeptide, as well as producing exogenous polypeptide in CHO-K1 and increasing specific productivity in CHO-K1. Method comprises culturing CHO-K1 in a culture medium containing a meta-tyrosine.
EFFECT: invention increases output of the exogenous polypeptide.
13 cl, 8 dwg, 1 tbl, 2 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PRODUCING RECOMBINANT POLYPEPTIDE | 2011 |
|
RU2577972C2 |
METHOD OF PRODUCING POLYPEPTIDES | 2015 |
|
RU2699715C2 |
IMPROVED CELL CULTURE MEDIUM | 2011 |
|
RU2798069C2 |
NOVEL EUKARYOTIC CELLS AND METHODS FOR PREPARING THEM FOR RECOMBINANT EXPRESSION OF A PRODUCT OF INTEREST | 2014 |
|
RU2720525C2 |
INSULIN ANALOGUE COMPLEX WITH REDUCED AFFINITY TO INSULIN RECEPTOR AND ITS USE | 2018 |
|
RU2779462C2 |
USE OF LOW TEMPERATURE AND/OR LOW pH IN CELL CULTURE | 2008 |
|
RU2478702C2 |
MEDIUM FOR CELLS CULTIVATION | 2011 |
|
RU2644651C2 |
METHOD FOR REDUCING ANTIBODY HETEROGENEITY AND METHOD FOR PRODUCING RELATED ANTIBODIES | 2012 |
|
RU2580020C2 |
HUMANIZED ANTIBODIES SPECIFIC TO ALPHA-ENOLASE AND METHODS FOR USE IN ANTITUMOR THERAPY | 2014 |
|
RU2761662C2 |
IMPROVED CELL CULTURE MEDIUM | 2011 |
|
RU2563353C2 |
Authors
Dates
2020-06-30—Published
2015-07-31—Filed