METHOD OF PREPARING COW BLOOD PLASMA SAMPLE FOR DETERMINING COMPOSITION OF FREE AMINO ACIDS Russian patent published in 2013 - IPC G01N30/06 G01N33/50 

FIELD: chemistry.

SUBSTANCE: disclosed is a method of preparing a cow blood plasma sample for determining composition of free amino acids by ion-exchange chromatography. 40-45 mg of sulphosalicylic acid are added to 1 ml of cow blood plasma, stirred for 2-3 minutes, centrifuged and the supernatant fluid is filtered. Lithium hydroxide is added to the cleaned filtrate in amount of 0.002-0.004 mg per ml of the cleaned filtrate and thoroughly shaken until complete dissolution.

EFFECT: invention improves the quality of isolation and accuracy of determining free amino acids from a cow blood plasma sample, reduces labour costs and time for all steps of the process.

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