FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to genetic engineering and biotechnology, and may be used in food and pharmaceutical industries. A microorganisms of the genus Corynebacterium producing 5'-inosinic acid is with provided with a capability to overexpress the genes encoding enzymes for the purine biosynthetic pathway. These enzymes include ribosephosphate-pyrophosphokinase, phosphoribosylglycinamide-formyltransferase, phosphoribosylformylglycinamidine synthetase, phosphoribosylformylglycinamidine synthetase II, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase, inosinic acid cyclohydrolase, phosphoribosylpyrophosphate-amidotransferase and phosphoribosylaminoimidazole synthetase. A method for preparing 5'-inosinic acid involves culturing this microorganism and recovering an end product from the culture medium.
EFFECT: use of the invention provides a high yield of 5'-inosinic acid.
12 cl, 7 dwg, 1 tbl, 2 ex
