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2 483 114

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(51)

IPC

C12Q1/68(2006-01-01)

(21) (22)

Application

2012117652/10, 2012-04-27

(24)

Start date

2012-04-27

(22)

Actual filing date

2012-04-27

(45)

Published

2013-05-27

(72)

Inventor

Potaturkina-Nesterova Natalija IosifovnaNemova Irina Sergeevna

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Obrazovatel'Noe Uchrezhdenie Vysshego Professional'Nogo Obrazovanija Gosudarstvennyj Universitet"

METHOD TO DETECT GENES THAT DETERMINE ADHESINS, HEMOLYSINS, MANNOSE-RESISTANT HAEMAGGLUTININS IN GENITAL STRAINS OF Escherichia coli Russian patent published in 2013 - IPC C12Q1/68

Abstract RU 2483114 C1

FIELD: biotechnologies.

SUBSTANCE: method is proposed, including extraction of a biomaterial, extraction of a bacterial DNA, amplification of DNA fragments in the examined strain by PCR method with application of a unique set of oligonucleotide primers, where products of amplification are separated by means of electrophoresis in agarose gel. The PCR results determine simultaneously all three genes, which are taken into account by visualisation through dyeing with etidium bromide. Availability of pathogenicity genes in the examined biomaterial in strains Escherichia coli is determined by dimensions of produced DNA fragments: fim A - 248 base pairs, hly A,B,C - 233 base pairs, pap C - 251 base pairs.

EFFECT: invention may be used in medicine to detect genital coliform bacilli with pathogenicity factors by method of molecular hybridisation.

2 tbl, 3 dwg

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RU 2 483 114 C1

Authors

Potaturkina-Nesterova Natalija Iosifovna

Nemova Irina Sergeevna

Dates

2013-05-27—Published

2012-04-27—Filed